The Physical Cell Biology Subgroup is hosting a virtual mini-symposium aimed at connecting early career researchers who apply physical principles to gain a deeper mechanistic understanding of cell biology at different spatiotemporal scales. This symposium will showcase presentations by young researchers who are utilizing theory, computation, and experiments to investigate the structure, dynamics, and interactions of cells in physiological contexts. The objective is to encourage collaborative interactions between theoretical and experimental approaches in order to advance the field. The talks are based on abstracts submitted for the Biophysical Society Annual Meeting in February 2025 that were not chosen for oral presentation but that carry the promise of creativity and vigor in applying physical principles to gain deeper mechanistic insights into cell biology. This event is part of the Physical Cell Biology Subgroup’s initiative to promote visibility, breadth, and networking opportunities for young researchers in this field.