Frontiers in Single-Molecule Biophysics is a webinar hosted by the Single-Molecule Force, Manipulation, Visualization Subgroup. This event will feature five exciting talks highlighting recent advances in single-molecule biophysics. The speakers will present cutting-edge applications of diverse single-molecule techniques to address fundamental biological questions, ranging from molecular mechanisms to complex biomolecular interactions. The webinar aims to bring together researchers and trainees interested in single-molecule methods and to showcase how these powerful approaches are transforming our understanding of biological systems.